By R. J. Hay (auth.), R. J. Hay MD, MRCP, MRCPath. (eds.)
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Excerpta Medica, Amsterdam, 129 5. Plempel M (1983) Antimycotic efficacy of bifonazole in vitro and in vivo. Arzneimittelforsch 33: 517 52 Bifonazole in Dermatological Practice: Results of a Multicentre Study F. Saffe BAYER AG, Pharma Deutschland, Medizinisch-Wissenschaftliche Abteilung, 5090 Leverkusen, Bayerwerk, Federal Republic of Germany Abstract The desire to improve the therapeutic possibilities open to dermatologists in private practice and GPs has drawn attention increasingly to the problem of patient compliance.
54 Patients and Methods This multi centre study was started directly after bifonazole had been licensed by the Federal Board of Health in the Federal Republic of Germany in 1983. It lasted 12 months, 1069 dermatologists in office practice took part and 6747 patients were enrolled in the study. In this connection, I would like to thank all of them for their cooperation in the present study. Approximately 68% (4560) of the 6747 case records submitted were evaluable with regard to efficacy. g. dermatophytes or yeasts) Diagnosis of infection with Malassezia furfur established by Wood's Light Criteria of exclusion from trial Negative mycological findings in the presence of positive clinical findings Topical or systemic antifungal treatment during 2 or 4 weeks prior to trial Concomitant treatment with corticosteroids Patients were admitted to the study only if positive findings upon direct microscopic examination of the wet smear were confirmed by positive cultural findings.
However, the routine procedures for mycological investigations including direct examinations are often very time consuming, complicated and not reliable, especially when • Blankophor P fliissig, produced by Bayer 32 hyphae are sparse in the specimen. A stain which is specific for fungi is still not available. New avenues for a simple and more reliable staining technique were opened with the use of fluorochromes and fluorescence microscopy. The method presented here differs from the earlier ones in the respect that a transparent tape is used both for the sampling and for the KOH mount and finally for the staining with the fluorochrome.